HTB-22 MCF7 [MCF-7] 人乳腺癌细胞

HTB-22 MCF7 [MCF-7] 人乳腺癌细胞，原代细胞|细胞系|细胞株|菌种，细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件！

HTB-22 MCF7 [MCF-7] 人乳腺癌细胞
ATCC® Number:  HTB-22™      
Designations:  MCF7 
Depositors:   CM McGrath 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Homo sapiens (human) 
Morphology: epithelial

 
Source: Organ: mammary gland; breast
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
Cell Type: epithelial
Cellular Products: insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5 
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Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Receptors: estrogen receptor, expressed
Antigen Expression: Blood Type O; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Cytogenetic Analysis: modal number = 82; range = 66 to 87.
The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic.
Isoenzymes:  AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1-2
PGM1, 1-2
PGM3, 1
Age:  69 years adult 
Gender:  female