Organ: peripheral blood Disease: plasmacytoma; myeloma Cell Type: B lymphocyte;
Cellular Products:
immunoglobulin
Permits/Forms:
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Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]
The cells produce, but do not secrete, immunoglobulin.
No surface immunoglobulin.
8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
Can be used as a human fusion line.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:
3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984 43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762 58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719
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[VIP第6年] 指数:6
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