CRL-1576 293 人胚肾细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|。细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件，
CRL-1576 293 人胚肾细胞
ATCC® Number: CRL-1576™
Designations: 12MBr6
Depositors: JL Caputo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: epithelial
Source: Organ: lung
Tissue: bronchus
Disease: normal
Cell Type: epithelial
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Isolation: Isolation date: July, 1979
Receptors: epidermal growth factor (EGF)
Cytogenetic Analysis: modal number = 62; range = 43 to 64.
The modal chromosome number was found in 78% of the cell population. The rate of polyploidy was 2.4%. Three consistent chromosome markers were identified: M1: del(1)9 ?; M2 del(13) and M3. Chromosome #20 is trisomic and the X chromosome is found in the disomic condition.
Age: 2 to 3 years
Gender: male
Comments: 12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green mon
key. This line was initiated in June, 1979 at the American Type Culture Collection from tissue explants using penicylinders and a trypsin-versene solution for cell dispersal. These cells contain PAS-positive cytoplasmic inclusions. Epidermal growth factor (EGF) was used to initiate and maintain growth. Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells. Progeny from the 12MBr6 were found to undergo at least 23 additional doublings beyond the frozen state.
Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The line requires epidermal growth factor as a supplement to the medium.
Subculturing: Protocol: .
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
