| Designations: |
SK-N-BE(2) |
| Depositors: |
JL Biedler |
| Biosafety Level: |
1 |
| Shipped: |
frozen |
| Medium & Serum: |
See Propagation |
| Growth Properties: |
mixed, adherent and suspension |
| Organism: |
Homo sapiens (human) |
| Morphology: |
neuroblast
|
| Source: |
Organ: brain
Disease: neuroblastoma
Derived from metastatic site: bone marrow |
| Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
| Restrictions: |
NOTE: SK-N-BE(2) was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. SK-N-BE(2) is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)SK-N-BE(2) or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of SK-N-BE(2) including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. |
| Isolation: |
Isolation date: November, 1972 |
| Tumorigenic: |
Yes |
| DNA Profile (STR): |
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6,7
TPOX: 8,11
vWA: 18 |
| Cytogenetic Analysis: |
modal number = 44; one or more HSR bearing chromosomes |
| Age: |
2 years |
| Gender: |
male |
| Comments: |
The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
The cells exhibit moderate levels of dopamine beta hydroxylase activity.
SK-N-BE(2) cells have a reported saturation density greater than 1 X 10 exp6 cells/sq cm.
The morphology of the cells varies with some cells having long processes and others that are epithelioid like.
The cells will aggregate, form clumps and float. |
| Propagation: |
ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Temperature: 37.0°C |
| Subculturing: |
Protocol: These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25 trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days |
| Preservation: |
Freeze medium: Complete growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase |
| Doubling Time: |
30 hrs |
| Related Products: |
recommended serum:ATCC 30-2020 |
| References: |
22535: Biedler JL, Spengler BA. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J. Natl. Cancer Inst. 57: 683-695, 1976. PubMed: 62055
22664: Barnes EN, et al. The fine structure of continuous human neuroblastoma lines SK-N-SH, SK- N-BE(2), and SK-N-MC. In Vitro 17: 619-131, 1981. PubMed: 7327593
22943: Biedler JL, Spengler BA. Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science 191: 185-187, 1976. PubMed: 942798
23032: Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704 |
