| Designations: |
MC/CAR |
| Depositors: |
Research Corporation |
| Isotype: |
IgG1 (cytoplasmic); kappa light chain |
| Biosafety Level: |
2 |
| Shipped: |
frozen |
| Medium & Serum: |
See Propagation |
| Growth Properties: |
suspension |
| Organism: |
Homo sapiens (human) |
| Morphology: |
lymphoblast
|
| Source: |
Organ: peripheral blood
Disease: plasmacytoma; myeloma
Cell Type: B lymphocyte; |
| Cellular Products: |
immunoglobulin |
| Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
| Receptors: |
complement (C3b) |
| Tumorigenic: |
Yes |
| Antigen Expression: |
HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 + |
| DNA Profile (STR): |
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 11,14
D7S820: 9,13
THO1: 6,9
TPOX: 8
vWA: 17,18 |
| Cytogenetic Analysis: |
normal male diploid |
| Isoenzymes: |
ADA, 1
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1 |
| Age: |
81 years |
| Gender: |
male |
| Ethnicity: |
Caucasian |
| Comments: |
Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]
The cells produce, but do not secrete, immunoglobulin.
No surface immunoglobulin.
8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
Can be used as a human fusion line. |
| Propagation: |
ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C |
| Subculturing: |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| References: |
3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719 |
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