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A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.
Derived from a buffy coat preparation from a patient with ALL.
The cells are EBNA positive.
Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10(5) viable cells/ml. Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967. 25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381
