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本商品的参数介绍如下：
Anti-Myc Tag Rabbit Monoclonal Antibody
PROPERTIES
|
Description |
Monoclonal rabbit antibody, cultured in vitro under conditions free from animal-derived components. |
|
Conjugate |
Unconjugated |
|
Purified By |
Affinity chromatography |
|
Antibody Product Type |
Primary antibody |
|
Concentration |
1mg/mL |
|
Isotype |
IgG1 |
|
Specificity |
Detects c-Myc p67 of mouse, rat, human, monkey, feline and canine origin, and recognizes c-Myc epitope tag at C-terminus of fusion proteins. |
|
Immunogen |
Recombinant protein encompassing the amino acids 408-439 within the C-terminal domain of human c-Myc |
STORAGE
|
Storage |
Store at -20°C, stable for one year. |
|
Storage Buffer |
PBS, pH 7.3, containing 0.02% sodium azide and 50% Glycerol |
APPLICATIONS
|
Species Reactivity |
human, mouse, rat, monkey, feline, canine |
|
Applications |
WB, IP, ELISA, IF, ChIP |
|
WB: 1:5000-1:20000 ELISA: 1:5000-1:20000 IP/ChIP: 2-4 μg /sample IF: 1:100-1:1000 Flow cytometry: 1 µg for 10e6 cells |
Western blot
Lysates at 10 µg per lane.
Lane 1: HEK-293T transfected with Myc tagged empty expression vector whole cell lysate (Negative control).
Lane 2: HEK-293T transfected with Myc tagged gene expression vector whole cell lysate.
Primary antibody: Anti-Myc Rabbit antibody (FI01105B) at 1/10000 dilution.
Secondary antibody: Goat Anti-Rabbitt IgG (HRP) at 1/8000 dilution.